| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 381 | Frankia casuarinae | AAAGCCCAACCTGCTCTCC | GTTCGTGTGACCAGGGGAT | 59.92 | 59.32 | 272 | 81 |
100.00%
|
100.00%
|
| Primer 382 | Frankia casuarinae | AATCAGGCTCCTGCACGAC | ACAACACCCGATCGCCAA | 60.08 | 59.57 | 179 | 80 |
100.00%
|
100.00%
|
| Primer 383 | Frankia casuarinae | ATCGGGTGTCATGCAGTCC | ACAACACCCGATCGCCAA | 59.78 | 59.57 | 209 | 80 |
100.00%
|
100.00%
|
| Primer 384 | Frankia casuarinae | GCAATCGGGTGTCATGCAG | ACAACACCCGATCGCCAA | 59.57 | 59.57 | 212 | 80 |
100.00%
|
100.00%
|
| Primer 385 | Frankia casuarinae | TCCGTTGGCCATGCTGAT | ATCAGCAGAACGCAGACCC | 59.32 | 60.08 | 168 | 80 |
100.00%
|
100.00%
|
| Primer 386 | Frankia casuarinae | GAACCAGAAGCGGATGGGA | TGTTCGATCCGGAGCAGGA | 59.40 | 60.68 | 288 | 80 |
100.00%
|
100.00%
|
| Primer 387 | Frankia casuarinae | GCAACTGGTGAGCCGTTCA | AGCTGGAGGCCATCGTCAA | 60.89 | 61.29 | 298 | 80 |
100.00%
|
100.00%
|
| Primer 388 | Frankia casuarinae | TTCGAGGAACGGTACCGGA | ACCGTGATGAGCGAGAACC | 60.30 | 59.78 | 197 | 80 |
100.00%
|
100.00%
|
| Primer 389 | Frankia casuarinae | TCCGACCTGATCAGTGTGG | GCATTCGCGTATCCGAGGA | 58.72 | 60.01 | 188 | 80 |
100.00%
|
100.00%
|
| Primer 390 | Frankia casuarinae | GGTCCGACCTGATCAGTGT | GCATTCGCGTATCCGAGGA | 58.72 | 60.01 | 190 | 80 |
100.00%
|
100.00%
|